John O'Neill


Affiliation: Medical Research Council-Laboratory of Molecular Biology (MRC-LMB), Cambridge, UK
E-mail address: oneillj@mrc-lmb.cam.ac.uk

John O'Neill studied Biochemistry as an undergraduate at the University of Oxford. This was followed by Ph.D. research on the mammalian circadian clock in the lab of Michael Hastings at the MRC Laboratory of Molecular Biology (LMB) in Cambridge. John continued to work on biological timing, next in plants and algae, with Andrew Millar at the University of Edinburgh. Finally, he worked with Akhilesh Reddy (University of Cambridge) on biological timing in human red blood cells. In 2013 he was recruited to become a group leader in the LMB's Cell Biology division. John has published 46 papers since 2003. The O'Neill lab's current research is focused on understanding the fundamental molecular mechanisms of the cellular clockwork, and how it facilitates temporal regulation of biological function.

Abstract

The Cellular Circadian Clock Drives Daily Rhythms of Ion Transport

Circadian clocks are fundamental to the biology of most organisms, coordinating physiology and behaviours (such as sleep) to resonate with the environmental cycle of day and night, through complex networks of clock-controlled genes. Circadian disruption in humans, as occurs during shift work, is strongly associated with a range of chronic diseases e.g. type II diabetes, various cancers. A fundamental knowledge gap exists, however, between clock gene expression cycles and the biochemical mechanisms that ultimately imparts circadian regulation to the activity of individual cells. Using a wide range of model organisms, including human, fungal and algal cells, as well as techniques such as dielectrophoresis and inductively-coupled plasma mass spectrometry, we have observed a daily rhythm in the import/export of K+ and Mg2+ions over the cell's plasma membrane. The striking evolutionary conservation of these daily rhythms of ion transport, combined with their functional consequences for cellular energy balance, suggests a fundamental yet unexplored feature of eukaryotic circadian timekeeping that has persisted from a common ancestor living >1.5 billion years ago.